discontinuous percoll gradient (Cytiva Europe)

Cytiva Europe is a verified supplier

Cytiva Europe manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cytiva Europe discontinuous percoll gradient
Discontinuous Percoll Gradient, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 12923 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percoll+gradient/Percoll/pm42263574-76-23-29
Average 99 stars, based on 12923 article reviews

discontinuous percoll gradient - by Bioz Stars, 2026-09

99/100 stars

Images

Centrifugation:

Article Title: Human hepatic stellate cells orchestrate the accumulation and function of CD103 + tissue-resident CD8 + T-cells in liver fibrosis
Article Snippet: After enzymatic digestion, tissue samples were subjected to mechanical disruption using the GentleMACS (Miltenyi Biotech) dissociator and debris removed by filtration through a 70 μM filter (Griener). .. Parenchymal cells were removed by centrifugation on a 30% Percoll gradient (GE Healthcare; VWR) followed by leukocyte isolation by a further round of density centrifugation using a Pancoll gradient (PAN Biotech). ..

Article Title: Neuroectodermal interleukin 12 signaling attenuates spinal cord inflammation and demyelination.
Article Snippet: Mechanical dissociation of digested tissue was carried out using a 19-gauge needle, followed by filtration of the resulting cell suspension through a 100 μm cell strainer (Bioswisstec). .. To further enrich the single-cell population from the spinal cord, a 30% Percoll gradient (GE Healthcare) was used, followed by centrifugation at 1,590 g for 30 minutes at 4°C without brakes. .. RNA was isolated from primary cultures using the RNA Isolation Kit (Servicebio, Wuhan, China) according to the manufacturer’s protocol.

Article Title: CGRP signaling links tumor-associated pain to immune evasion in oral squamous cell carcinoma
Article Snippet: Briefly, tissue harvested into HBSS+H, minced and subjected to a two-step enzymatic digestion consisting of L-cysteine and papain (Sigma-Aldrich) for 10 min at 37°C followed by a combined collagenase type II and dispase II (Gibco) digestion for 20 min at 37°C. .. Tissue was then centrifuged at 240xg to pellet and passed through a Percoll gradient (1000xg centrifugation, GE Healthcare) to isolate neurons and resuspended in DMEM/F12. .. For functional assays, neurons were plated on 12mm poly-L-ornithine and laminin (Sigma-Aldrich) coated glass coverslips.

Article Title: Active LXR signaling, coupled with elevated mitochondrial and glycolytic metabolism contributes to GM-CSF–induced trained immunity
Article Snippet: .. Monocyte isolation was performed by two-step differential density centrifugation over Histopaque ® 1077 (Sigma-Aldrich, St. Louis, MO, USA, #10771) and a hyper-osmotic Percoll gradient (46% Percoll in RPMI, GE Healthcare, Uppsala, Sweden, #17089101). .. Cells were purified further with MACS Pan Monocyte Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany, #130-096-537) and washed once with serum-free RPMI-1640 medium before resuspension in complete RPMI culture medium.

Isolation:

Single Cell:

Suspension:

Article Title: First Glance at Myeloid Leukaemia Factor 2 in Cardiomyocytes
Article Snippet: Ventricles were isolated, minced, and digested in collagenase type II (0.5 mg/mL, Worthington Biochemical Corporation, Lakewood, NJ, USA) and pancreatin (0.6 mg/mL, Sigma-Aldrich, St. Louis, MO, USA) in sterile ADS buffer at 37 °C in four to five sequential digestion cycles until sufficient cell dissociation was achieved. .. The cell suspension was subsequently separated on a Percoll gradient (GE Healthcare, Chicago, IL, USA) to enrich cardiomyocytes and remove fibroblasts. .. Cardiomyocytes were plated in DMEM supplemented with 10% ( v / v ) foetal calf serum, 100 U/mL penicillin, 100 μg/mL streptomycin, and 2 mmol/L L-glutamine (Life Technologies, Carlsbad, CA, USA) for 24 h. After washing with warm PBS, cells were infected with adenoviral (Ad) vectors in serum-free medium.

Article Title: The RORγt ligand-binding domain controls the pathogenicity of IL-17A + T cells differently in autoimmune diseases of the skin and CNS.
Article Snippet: Tissues were lysed in RPMI 1640 medium supplemented with DNase I (200μg/mL; Sigma-Aldrich), homogenized with a syringe plunger, and filtered through a 70-μm cell strainer. .. Mononuclear cells were separated from the suspension by using a Percoll gradient (GE Healthcare). ..

Review